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sigmoidal dose–response fitting in graphpad prism software  (GraphPad Software Inc)


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    GraphPad Software Inc sigmoidal dose–response fitting in graphpad prism software
    Sigmoidal Dose–Response Fitting In Graphpad Prism Software, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sigmoidal+dose%E2%80%93response+fitting+in+graphpad+prism+software/sigmoidal+dose+response+curve/pmc11940079-124-12-12
    Average 90 stars, based on 1 article reviews
    sigmoidal dose–response fitting in graphpad prism software - by Bioz Stars, 2026-09
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    Article Title: Identification of a Possible Endocannabinoid-Mediated Mechanism of Action of Cetylated Fatty Acids
    Article Snippet: The data are presented as an average of 3 replicates (mean ± standard deviation), and a statistical analysis was performed using GraphPad Prism software. .. The IC 50 value was calculated using the Sigmoidal dose–response fitting in GraphPad Prism software. ..



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    Identification of peptides containing a potential AIM sequence in TgAtg3 by AlphaScreen competition assay. A, schematic of the AlphaScreen assay. Biotinylated TgAtg8 and His-tag TgAtg3 are immobilized on streptavidin-coated donor beads and nickel-chelated acceptor beads, respectively. Excitation of donor beads produces singlet oxygen. Interaction between TgAtg8 and TgAtg3 brings donor and acceptor beads into close proximity, allowing energy from singlet oxygen to be transferred to the acceptor beads and produce a light signal. Addition of untagged competitor peptides will reduce the signal intensity. B, competition for binding to biotinylated TgAtg8 domain by untagged peptides of TgAtg3. Each competitor was tested in triplicate, with a representative data set shown. IC50 values were calculated using a nonlinear regression analysis (sigmoidal dose–response fitting with variable slope (four parameters)) using GraphPad Prism 6.0 software. Data were obtained from one of four independent experiments.
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    Identification of peptides containing a potential AIM sequence in TgAtg3 by AlphaScreen competition assay. A, schematic of the AlphaScreen assay. Biotinylated TgAtg8 and His-tag TgAtg3 are immobilized on streptavidin-coated donor beads and nickel-chelated acceptor beads, respectively. Excitation of donor beads produces singlet oxygen. Interaction between TgAtg8 and TgAtg3 brings donor and acceptor beads into close proximity, allowing energy from singlet oxygen to be transferred to the acceptor beads and produce a light signal. Addition of untagged competitor peptides will reduce the signal intensity. B, competition for binding to biotinylated TgAtg8 domain by untagged peptides of TgAtg3. Each competitor was tested in triplicate, with a representative data set shown. IC50 values were calculated using a nonlinear regression analysis (sigmoidal dose–response fitting with variable slope (four parameters)) using GraphPad Prism 6.0 software. Data were obtained from one of four independent experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: Characterization of the molecular mechanism of the autophagy-related Atg8–Atg3 protein interaction in Toxoplasma gondii

    doi: 10.1074/jbc.RA118.002614

    Figure Lengend Snippet: Identification of peptides containing a potential AIM sequence in TgAtg3 by AlphaScreen competition assay. A, schematic of the AlphaScreen assay. Biotinylated TgAtg8 and His-tag TgAtg3 are immobilized on streptavidin-coated donor beads and nickel-chelated acceptor beads, respectively. Excitation of donor beads produces singlet oxygen. Interaction between TgAtg8 and TgAtg3 brings donor and acceptor beads into close proximity, allowing energy from singlet oxygen to be transferred to the acceptor beads and produce a light signal. Addition of untagged competitor peptides will reduce the signal intensity. B, competition for binding to biotinylated TgAtg8 domain by untagged peptides of TgAtg3. Each competitor was tested in triplicate, with a representative data set shown. IC50 values were calculated using a nonlinear regression analysis (sigmoidal dose–response fitting with variable slope (four parameters)) using GraphPad Prism 6.0 software. Data were obtained from one of four independent experiments.

    Article Snippet: IC 50 values were calculated using a nonlinear regression analysis (sigmoidal dose–response fitting with variable slope (four parameters)) using GraphPad Prism 6.0 software.

    Techniques: Sequencing, Amplified Luminescent Proximity Homogenous Assay, Competitive Binding Assay, Binding Assay, Software